Yay for lists of lesbian movies! Hehehe. I too have aquired several since I've moved out of the house... it's a lot easier to get ahold of this stuff without mommy and daddy looking over my shoulder

So far I have:
Imagine Me & You: It's Piper Perabo and Lena Headey. Together. So. Hot. OMG. Hahaha. It's a sweet story, and it's got some good kissage. I wish they would've done more with their relationship, but I understand it was just a romantic comedy. Still a wonderful movie.
Lost and Delirious: This was the first lesbian movie I ever bought, and will therefore always be close to my heart. This was during my Once and Again days where Jessie (Evan Rachel Wood) and Katie (Mischa Barton) were my two favorite people. *Sigh* I was so young then. I think the cashier was flirting with me when I bought it, but I was too young, stupid, and nervous to realize it.
Loving Annabelle: Dude. Erin Kelly is so freaking hot. Seriously. Katherine Brooks is a wonderful director, and I can't wait to see what else she comes out with (though I agree with you, WhatsThatSmell, the story could've been a whole lot better... I didn't even like the alternate ending either)
D.E.B.S.: Jordana Brewster as a lesbian. OMG. Wow, I so love her.
But I'm a Cheerleader: Just discovered this one a few months ago, after watching the Buffy the Vampire Slayer episode with Clea Duvall. She's so great in that movie. It's kinda lame, but incredibly sweet.
Buffy the Vampire Slayer seasons 4-6: I absolutely love the Willow/Tara storyline. I really don't think that any show/movie to date has had such a wonderful lesiban storyline (ok, so it was great until the middle of season 6, but still). They did a pretty good job with it, and it was one of the first shows to portray the lesbians as girly girls, not butch. I absolutely love Alyson Hannigan and Amber Benson!
Fingersmith: A little known movie that was a BBC adaptation of Sarah Water's book of the same title. If y'all get a chance, you should definately look it up!
That's all for now!